NPR1 gene transformation as assessed by germ cell in situ transformation pathway into Siraitia grosvenorii
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چکیده
منابع مشابه
Npr1 Gene Transformation as Assessed by Germ Cell in Situ Transformation Pathway into Siraitia Grosvenorii
NPR1 gene was transformed into Siraitia grosvenorii (Swingle) C. Jeffrey ex. A.M. Lu and Zhi Y. Zhang by germ cell in situ transformation. Ovary injection, cutting chapiter, and chapiter spreading treatments were applied in this study. The transgenic plants were selected using hygromycin screening and confirmed by PCR testing, genome integrated with NPR1 gene in transgenic plants was analyzed b...
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Continuous phytochemical studies of the crude extract of Luo Han Guo (Siraitia grosvenorii) furnished three additional new cucurbitane triterpene glycosides, namely 11-deoxymogroside V, 11-deoxyisomogroside V, and 11-deoxymogroside VI. The structures of all the isolated compounds were characterized on the basis of extensive NMR and mass spectral data as well as hydrolysis studies. The complete ...
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CYP450 plays an essential role in the development and growth of the fruits of Siraitia grosvenorii. However, little is known about the SgCYP450-4 gene in S. grosvenorii. Here, based on transcriptome data, a full-length cDNA sequence of SgCYP450-4 was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends (RACE) strategies. SgCYP450-4 is 1677 bp i...
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ژورنال
عنوان ژورنال: Bangladesh Journal of Botany
سال: 2018
ISSN: 2079-9926,0253-5416
DOI: 10.3329/bjb.v44i2.38513